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| Registros recuperados : 33 | |
| 3. |  | BATISTA, J. S. S.; RODRIGUES, E. P.; HUNGRIA, M. Extração de proteínas de raízes de soja para análise proteômica. In: CONGRESSO BRASILEIRO DE SOJA, 5.; MERCOSOJA 2009, Goiânia. Anais... Londrina: Embrapa Soja, 2009. Seção trabalhos, t. 172. 1 CD-ROM. Editado por Adilson de Oliveira Júnior, Odilon Ferreira Saraiva, Clara Beatriz Hoffmann Campo, César de Castro.| Biblioteca(s): Embrapa Soja. |
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| 5. | | SCHIAVON, A. L.; RODRIGUES, E. P.; BATISTA, J. S. S.; GOMES, D. F.; HUNGRIA, M. Análise proteômica de raízes de feijão (Phaseolus vulgaris) inoculadas com Rhizobium tropici. In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 5., 2010, Londrina. Resumos... Londrina: Embrapa Soja, 2010. p. 9-12. (Embrapa Soja. Documentos, 323). Editado por Odilon Ferreira Saraiva, Paula Geron Saiz Melo.| Biblioteca(s): Embrapa Soja. |
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| 6. | | OLIVEIRA, L. R.; MARCELINO, F. C.; BARCELLOS, F. G.; RODRIGUES, E. P.; MEGIAS, M.; HUNGRIA, M. The nodC, nodG, and glgX genes of Rhizobium tropici strain PRF 81. Functional & Integrative Genomics, Heidelberg, v. 10, n. 3, p. 425-431, Aug. 2010.| Biblioteca(s): Embrapa Soja. |
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| 7. | | BATISTA, J. S. da S.; RODRIGUES, E. P.; TORRES, A. R.; GOMES, D. F.; HUNGRIA, M. Efeito da inoculação com Bradyrhizobium japonicum no proteoma de raízes de soja. In: CONGRESSO BRASILEIRO DE MICROBILOGIA, 26.; SIMPÓSIO INTERNACIONAL DE BACTÉRIAS LÁTICAS; ENCONTRO NACIONAL DE PROFESSORES DE MICROBIOLOGIA; SIMPÓSIO DE COLEÇÕES DE CULTURAS, 4., 2011, Foz do Iguaçu. Anais... São Paulo: SBM, 2011. Resumo, 776-1.| Biblioteca(s): Embrapa Soja. |
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| 9. | | OLIVEIRA, L. R.; RODRIGUES, E. P.; MARCELINO-GUIMARÃES, F. C.; OLIVEIRA, A. L. M.; HUNGRIA, M. Fast induction of biosynthetic polysaccharide genes lpxA, lpxE, and rkpI of Rhizobium sp. strain PRF 81 by common bean seed exudates is indicative of a key role in symbiosis. Functional & Integrative Genomics, v. 13, n. 2, p. 275-283, Jun. 2013.| Biblioteca(s): Embrapa Soja. |
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| 10. | | RODRIGUES, E. P.; BATISTA, J. S. S.; TORRES, A. R.; HUNGRIA, M. Extração de proteínas de raízes de soja para análise proteômica. In: CONGRESSO BRASILEIRO DE SOJA, 5.; MERCOSOJA 2009, Goiânia. Resumos... Londrina: Embrapa Soja, 2009. p. 102, trab. 172. Editado por Adilson de Oliveira Júnior, Odilon Ferreira Saraiva, Clara Beatriz Hoffmann Campo, César de Castro.| Biblioteca(s): Embrapa Soja. |
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| 11. | | BARCELLOS, F. G.; ARAÚJO, T. G. F. de; RODRIGUES, E. P.; HUNGRIA, M. Taxonomic identification and molecular phylogeny of rhizobia isolates that nodulate bean from Paraná soils. In: BRAZILIAN CONGRESS OF GENETICS, 66.; SIMPÓSIO DE CITOGENÉTICA E GENÉTICA DE PEIXES, 19.; REUNIÃO DE GENÉTICA DE MICRORGANISMOS, 32.; CONGRESSO LATINO-AMERICANO DE GENÉTICA PARA CONSERVAÇÃO, 2., 2021, online. Abstracts... Ribeirão Preto: SBG, 2021. e-book. p. 365.| Biblioteca(s): Embrapa Soja. |
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| 12. | | RODRIGUES, E. P.; TORRES, A. R.; BATISTA, J. S. da S. B.; HUERGO, L.; HUNGRIA, M. A simple, economical and reproducible protein extraction protocol for proteomics studies of soybean roots. Genetics and Molecular Biology, Ribeirão Preto, v. 35, n. 1, suppl., p. 348-352, May 2012.| Biblioteca(s): Embrapa Soja. |
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| 14. | | RODRIGUES, E. P.; COELHO, M. S.; SOARES, C. de P.; VIDAL, M. S.; ARAÚJO, J. L. S.; BALDANI, J. I. Identificação e quantificação de compostos indólicos no sobrenadante de Gluconacetobacter dizaotrophicus, cultivada em meio liquido, por cromatografia líquida de alta eficiência (HPLC). Seropédica: Embrapa Agrobiologia, 2009. 25 p. (Embrapa Agrobiologia. Boletim de pequisa e desenvolvimento, 52).| Biblioteca(s): Embrapa Unidades Centrais. |
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| 16. | | RODRIGUES, E. P.; SOARES, C. P.; OLIVEIRA, A. L. M.; VIDAL, M. S.; SIMÕES-ARAÚJO, J. L.; BALDANI, J. I. Mutantes de Gluconacetobacter diazotrophicus (estirpe PAL5) no gene que codifica um amino oxidase são deficientes na biossíntese de ácido indol-3-acético. In: CONGRESSO BRASILEIRO DE GENÉTICA, 53., 2007, Águas de Lindóia, SP. Resumos... Águas de Lindóia: SBG, 2007. 1 p. 1 CD-ROM.| Biblioteca(s): Embrapa Agrobiologia. |
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| 17. | | GALVÃO, P. Q.; RODRIGUES, E. P.; ROUWS, L. F. M.; ARAUJO, J. L. S. de; VIDAL, M. S.; BALDANI, J. I. Analysis of Gluconacetobacter diazotrophicus PAL5 nitB mutant. In: INTERNATIONAL SYMPOSIUM ON BIOLOGICAL NITROGEN WITH NON-LEGUMES, 12.; INTERNATIONAL INCT SYMPOSIUM ON NITROGEN BIOLOGICAL FIXATION, 2., 03 a 8 de outubro, 2010, Búzios, RJ. Book of abstracts... Seropédica: Embrapa Agrobiologia, 2010. SI 13| Biblioteca(s): Embrapa Agrobiologia. |
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| 19. | | GALVÃO, P. G.; RODRIGUES, E. P.; ROUWS, L. F. M.; ARAUJO, J. L. S. de; VIDAL, M. S.; BALDANI, J. I. Geração e avaliação de um mutante nitB de Gluconacetobacter diazotrophicus. In: SEMANA CIENTÍFICA JOHANNA DÖBEREINER, 10., 18 a 24 de outubro de 2010, Seropédica. Ciência para o Desenvolvimento Sustentável: anais... Seropédica: Embrapa Agrobiologia, 2010.| Biblioteca(s): Embrapa Agrobiologia. |
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| 20. | | RODRIGUES, E. P.; COELHO, M. S.; SOARES, C. de P.; VIDAL, M. S.; ARAUJO, J. L. S. de; BALDANI, J. I. Identificação e quantificação de compostos indólicos no sobrenadante de Gluconacetobacter diazotrophicus, cultivada em meio liquido, por cromatografia líquida de alta eficiência (HPLC). Seropédica: Embrapa Agrobiologia, 2009. 25 p. (Embrapa Agrobiologia. Boletim de Pesquisa e Desenvolvimento, 52).| Biblioteca(s): Embrapa Agrobiologia. |
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| Registros recuperados : 33 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
11/07/2013 |
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Data da última atualização: |
02/08/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
OLIVEIRA, L. R.; RODRIGUES, E. P.; MARCELINO-GUIMARÃES, F. C.; OLIVEIRA, A. L. M.; HUNGRIA, M. |
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Afiliação: |
LUCIANA RUANO OLIVEIRA, UEL; ELISETE PAINS RODRIGUES, UEL; FRANCISMAR CORREA MARCELINO-GUIMARÃES, CNPSO; ANDRÉ LUIZ MARTINEZ OLIVEIRA, UEL; MARIANGELA HUNGRIA, CNPSO. |
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Título: |
Fast induction of biosynthetic polysaccharide genes lpxA, lpxE, and rkpI of Rhizobium sp. strain PRF 81 by common bean seed exudates is indicative of a key role in symbiosis. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Functional & Integrative Genomics, v. 13, n. 2, p. 275-283, Jun. 2013. |
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ISSN: |
1438-7948 |
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DOI: |
10.1007/s10142-013-0322-7 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Rhizobial surface polysaccharides (SPS) are, together with nodulation (Nod) factors, recognized as key molecules for establishment of rhizobia?legume symbiosis. In Rhizobium tropici, an important nitrogen-fixing symbiont of common bean (Phaseolus vulgaris L.), molecular structures and symbiotic roles of the SPS are poorly understood. In this study, Rhizobium sp. strain PRF 81 genes, belonging to the R. tropici group, were investigated: lpxA and lpxE, involved in biosynthesis and modification of the lipid-A anchor of lipopolysaccharide (LPS), and rkpI, involved in synthesis of a lipid carrier required for production of capsular polysaccharides (KPS). Reverse transcription quantitative PCR (RT-qPCR) analysis revealed, for the first time, that inducers released from common bean seeds strongly stimulated expression of all three SPS genes. When PRF 81 cells were grown for 48 h in the presence of seed exudates, twofold increases (p < 0.05) in the transcription levels of lpxE, lpxA, and rkpI genes were observed. However, higher increases (p < 0.05) in transcription rates, about 50-fold for lpxE and about 30-fold for lpxA and rkpI, were observed after only 5 min of incubation with common bean seed exudates. Evolutionary analyses revealed that lpxA and lpxE of PRF81 and of the type strain of R. tropici CIAT899Tclustered with orthologous Rhizobium radiobacter and were more related to R. etli and Rhizobium leguminosarum, while rkpI was closer to the Sinorhizobium sp. group. Upregulation of lpxE, lpxA, and rkpI genes suggests that seed exudates can modulate production of SPS of Rhizobium sp. PRF81, leading to cell wall changes necessary for symbiosis establishment. MenosAbstract: Rhizobial surface polysaccharides (SPS) are, together with nodulation (Nod) factors, recognized as key molecules for establishment of rhizobia?legume symbiosis. In Rhizobium tropici, an important nitrogen-fixing symbiont of common bean (Phaseolus vulgaris L.), molecular structures and symbiotic roles of the SPS are poorly understood. In this study, Rhizobium sp. strain PRF 81 genes, belonging to the R. tropici group, were investigated: lpxA and lpxE, involved in biosynthesis and modification of the lipid-A anchor of lipopolysaccharide (LPS), and rkpI, involved in synthesis of a lipid carrier required for production of capsular polysaccharides (KPS). Reverse transcription quantitative PCR (RT-qPCR) analysis revealed, for the first time, that inducers released from common bean seeds strongly stimulated expression of all three SPS genes. When PRF 81 cells were grown for 48 h in the presence of seed exudates, twofold increases (p < 0.05) in the transcription levels of lpxE, lpxA, and rkpI genes were observed. However, higher increases (p < 0.05) in transcription rates, about 50-fold for lpxE and about 30-fold for lpxA and rkpI, were observed after only 5 min of incubation with common bean seed exudates. Evolutionary analyses revealed that lpxA and lpxE of PRF81 and of the type strain of R. tropici CIAT899Tclustered with orthologous Rhizobium radiobacter and were more related to R. etli and Rhizobium leguminosarum, while rkpI was closer to the Si... Mostrar Tudo |
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Thesagro: |
Feijão. |
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Categoria do assunto: |
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Marc: |
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245 $aFast induction of biosynthetic polysaccharide genes lpxA, lpxE, and rkpI of Rhizobium sp. strain PRF 81 by common bean seed exudates is indicative of a key role in symbiosis.$h[electronic resource]
260 $c2013
520 $aAbstract: Rhizobial surface polysaccharides (SPS) are, together with nodulation (Nod) factors, recognized as key molecules for establishment of rhizobia?legume symbiosis. In Rhizobium tropici, an important nitrogen-fixing symbiont of common bean (Phaseolus vulgaris L.), molecular structures and symbiotic roles of the SPS are poorly understood. In this study, Rhizobium sp. strain PRF 81 genes, belonging to the R. tropici group, were investigated: lpxA and lpxE, involved in biosynthesis and modification of the lipid-A anchor of lipopolysaccharide (LPS), and rkpI, involved in synthesis of a lipid carrier required for production of capsular polysaccharides (KPS). Reverse transcription quantitative PCR (RT-qPCR) analysis revealed, for the first time, that inducers released from common bean seeds strongly stimulated expression of all three SPS genes. When PRF 81 cells were grown for 48 h in the presence of seed exudates, twofold increases (p < 0.05) in the transcription levels of lpxE, lpxA, and rkpI genes were observed. However, higher increases (p < 0.05) in transcription rates, about 50-fold for lpxE and about 30-fold for lpxA and rkpI, were observed after only 5 min of incubation with common bean seed exudates. Evolutionary analyses revealed that lpxA and lpxE of PRF81 and of the type strain of R. tropici CIAT899Tclustered with orthologous Rhizobium radiobacter and were more related to R. etli and Rhizobium leguminosarum, while rkpI was closer to the Sinorhizobium sp. group. Upregulation of lpxE, lpxA, and rkpI genes suggests that seed exudates can modulate production of SPS of Rhizobium sp. PRF81, leading to cell wall changes necessary for symbiosis establishment.
650 $aFeijão
700 1 $aRODRIGUES, E. P.
700 1 $aMARCELINO-GUIMARÃES, F. C.
700 1 $aOLIVEIRA, A. L. M.
700 1 $aHUNGRIA, M.
773 $tFunctional & Integrative Genomics$gv. 13, n. 2, p. 275-283, Jun. 2013.
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